Reshetov I.V., Samoilova S.I., Sukortseva N.S., Gaboyan A.G., Svyatoslavov D.S., Put V.A., Kudrin K.G. In vivo modeling of a prefabricated cartilaginous autograft for reconstruction of the auricle in an experiment. Golova i sheya. Rossijskij zhurnal Head and neck Russian Journal. 2020;8(1):8–14 (in Russian).
DOI: https://doi.org/10.25792/HN.2020.8.1.8-14
Otoplasty is a highly eligible operation for congenital conditions, injuries, iatrogenic deformities, and tumors. Secondary otoplasty is the most complex operation type requiring the use of supportive plastic material. The search for the optimal plastic material for cartilage reconstruction led to conclusion that autografts have the best engraftability, however, demonstrating an efficiency of less than 70%, and in addition, the difficulty of restoring the exact shape of the ear cartilage necessitates a search for improved secondary otoplasty technique. The aim of the study. An experiment is proposed to find an effective method for reconstruction of the ear cartilage based on cartilage autograft in the form of micrografts enclosed in a plastic scaffold made of polytetrafluoroethylene.
Material and methods. Tubular scaffolds are made in the forms of a continuous tube and a tube with perforations. The engraftment rate of 60 scaffolds was studied, 30 in each group.
Results. The results showed effective vascular invasion and active living cartilage tissue (proliferation index was more than 30% of cells) in more than 90% cases in the group with a perforated scaffold, while in the group with a continuous scaffold, cartilage engraftment was achieved only in a limited part of autografts, in the center aseptic lysis of autografts occurred. A discussion of the results suggests the possibility of successful reconstruction of the supporting cartilage using micrografts enclosed in a plastic scaffold shape, which initially retains its volume and shape, without proper hardness, and as the vessels grow and the chondrocytes nutrition is restored, the needed supportive strength is achieved.
Conclusion. Studies of the use of a perforated scaffold made of a biocompatible plastic material, in which micrografts of cartilage autotransplant are placed, have demonstrated the restoration of supportive ability in secondary otoplasty.
Key words: otoplasty, cartilage, autograft, scaffold, micrograft
Conflicts of interest. The authors have no conflicts of interest to declare. Funding. The work was supported by the grant of RFBR 15-29-04819.
For citation: Reshetov I.V., Samoilova S.I., Sukortseva N.S., Gaboyan A.G., Svyatoslavov D.S., Put V.A., Kudrin K.G. In vivo modeling of a prefabricated cartilaginous autograft for reconstruction of the auricle in an experiment. Golova i sheya. Rossijskij zhurnal Head and neck Russian Journal. 2020;8(1):8–14 (in Russian).
The authors are responsible for the originality of the data presented and the possibility of publishing illustrative material – tables, figures, photographs of patients.