For citation:

Balatskaya N.V., Filatova I.A., Kulikova I.G., Denisyuk V.O. Features of the systemic production of pro-inflammatory and chemoattractant mediators in patients with persisting chronic post-traumatic uveitis. Head and neck. Russian Journal. 2021;9(2):43–48 (In Russian).

The authors are responsible for the originality of the data presented and the possibility of publishing illustrative material – tables, figures, photographs of patients.

Doi: 10.25792/HN.2021.9.2.43–48

Purpose of the study. To assess the content of pro-inflammatory and chemoattractant mediators in the peripheral blood in patients with persisting chronic post-traumatic uveitis.

Material and methods. We examined 150 patients, most of which were male (59.9%), with the eye trauma outcomes (including surgical trauma). The chronic post-traumatic uveitis duration ranged from 11 months to 21 years after injuries or ophthalmic surgery. The patients were divided into four groups depending on the traumatic impact type. Group I consisted of 57 people (57 eyes) after a penetrating injury of the eyeball, without long-term surgical interventions. Group II included 53 patients (53 eyes) with the outcome of an eye contusion injury without rupture or prolapse of membranes, without surgical interventions in the early and long-term period. Group III included 29 patients with multiple ophthalmic surgical interventions. Group IV consisted of 11 patients (11 eyes) with the outcome of a single intraocular surgery. Results. The multiplex analysis results demonstrated the presence of 11 out of 20 cytokines studied in the blood serum of patients with chronic post-traumatic uveitis: SDF-1α, RANTES, TGF-β1 in 100% of cases, and EOTAXIN, IP- 10, MCP-1, MIP-1β in 90% of blood serum samples. It should be noted that we detected the pro-inflammatory cytokines IL-18, IL-8, and inducible chemoattractant GRO-α, which were absent in the control group blood samples, in almost one third of patients with chronic post-traumatic uveitis. Comparative analysis of the concentrations of the studied mediators in blood serum revealed significant differences with the control group: in EOTAXIN levels for groups II and III, in MIP -1α level for group I, in SDF-1α, RANTES, and TGF-β1 for all studied groups. We found a statistically significant increase in the serum EOTAXIN content in groups II (in the outcome of contusion injury) and III (in the outcome of multiple surgical interventions) – 75.9±4.08 and 72.2±8.03 pg / ml, respectively. The levels of TGF-β1 in the blood serum of all patients with chronic posttraumatic uveitis were similar and significantly exceeded the value of this cytokine in the blood serum of healthy donors.

Conclusion. Thus, long-term chronic post-traumatic uveitis, regardless of the type of injury, is associated with increased systemic production of pro-inflammatory, chemoattractant mediators IL-18, EOTAXIN, GRO-A, IP-10, MCP-1, MIP-1α, MIP-1β, SDF-1α, RANTES, IL-8, with the addition of a compensatory anti-inflammatory response (an increase in the level of TGF-β1 in the blood cells of patients of all studied groups). The most significant shifts were determined for IL-8, IL-18, TGF-β1 levels.

Key words: sympathetic ophthalmia, post-traumatic uveitis, chemokines, cytokines, inflammation, vitreoretinal surgery

Conflicts of interest. The authors have no conflicts of interest to declare. Funding. There was no funding for this study.

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