Kastyro I.V., Ibadullaeva S.S., Lavrentyeva E.A., Ganshin I.B., Khlystalov M.V., Moroz S.E., Korolev A.G., Kartasheva A.F., Barannik M.I., Sarygin P.V., Popadyuk V.I., Kostyaeva M.G. Changes in the morphofunctional state of sebaceous glands in rats after fat autotransplantation in rats at late stages. Head and neck. Russian Journal. 2025;13(2):29–36
DOI: https://doi.org/10.25792/HN.2025.13.2.29-36
The quality of transplanted fat depends largely on the type of fat tissue processing before its transplantation. Lipofilling is necessary to eliminate the deficit of tissue volume; there is a problem of its engraftment in the recipient site. Insufficient attention is paid in clinical and experimental studies to the study of the reactions of the skin and its appendages (hair follicles, sebaceous glands, etc.) during fat autotransplantation after various methods of its processing. There are no works aimed at studying the activity of such a proliferation marker as Ki-67 in the sebaceous glands during fat autotransplantation.
Aims: to evaluate changes in the morphofunctional state of the sebaceous glands and the expression of Ki-67 as a proliferation marker in the sebaceous glands in rats after fat autotransplantation in rats at late stages.
Material and methods. The expression of the Ki-67 bek in the sebaceous glands after fat autotransplantation in rats was studied after 30, 90 and 180 days. Three types of fat autografts were used: solid graft, scalpel-cut graft, and homogenized fat in a Luer Lock syringe. Results. Fat autografting stimulates the expression of Ki-67 protein by cells in the terminal sections of the sebaceous glands, with this indicator being higher in the solid graft and homogenized fat groups after a month, and in the scalpelcut fat graft group after 3 and 6 months. The higher the expression of Ki-67 protein by sebaceous gland cells, the larger their area, indicating different effects of some methods of preoperative treatment of fat autografts on their morphofunctional activity at the site of fat transplantation. Transplantation of small fat grafts at 3 and 6 months in the recipient area has a greater similar effect.
Conclusion. The side effect of homogenized fat autografting into the hypodermis at late stages may have a therapeutic effect in reducing the proliferative activity of the sebaceous glands.
Key words: fat transplantation, Ki-67, fat grafting, proliferation, sebaceous glands.
Conflicts of interest. The authors have no conflicts of interest to declare. Funding. There was no funding for this study