Hemocapillary diameter after fat grafting at late stages in biological objects

Aims: to evaluate the diameter of microcapillaries in the vascular bed after fat grafting in rats in the late postoperative period. Material and methods. A morphometric assessment of the diameter of hemocapillaries and VEGF-A expression was performed after fat grafting in rats. The fat transplantation area was stained with rabbit monoclonal antibodies to VEGF-A and counterstained with Mayer’s hematoxylin. Results. In small fat grafts, as well as in solid grafts, the intensity of VEGF-a protein expression 30 days after grafting was higher in the graft center. The diameter of working hemocapillaries was wider in solid and crushed grafts than in the hypodermis after the introduction of homogenized fat. Conclusion. At three months post-lipofilling, an active process of neoangiogenesis occurs at the periphery of small fat grafts, which may indirectly indicate more intense integration of small fat grafts into recipient tissues.
Key words: VEGF-A, autologous fat transplantation, lipofilling, fat grafts, hemocapillary, angiogenesis, hypoxia, necrosis, ischemia, arterioles, venules
Conflict of interest. The authors have no conflicts of interest.
Funding. The study was conducted without sponsorship.